O-chem vs. Biochem – oh so related, yet oh so different!
Whether you loved o-chem (which I hope you did) or hated it, you still might love biochemistry! O-chem is at […]
Biochemistry things worth memorizing
I’m not a fan of rote memorization, but there are some things I do recommend (and for my class(es) will […]
Key biochemistry equations
Here’s an overview of what I feel are the key biochemistry equations to know (including thermodynamics, Henderson-Hasselbalch, and Michaelis-Menten). I […]
Packing protein chromatography columns
As a biochemist, you might have thousands of CVs! CV as in “column volume” that is! For affinity chromatography, this […]
Quick tip: use interlibrary loan to access materials you think you can’t access
Libraries and underutilized- especially interlibrary loan! Libraries basically have an insiders network where they share material with one another – […]
Practical protein purification tip: easily & quickly monitor protein elution by eye using Bradford dye
Quick protein purification tip: when doing gravity flow chromatography, monitor protein elution by dye by checking fractions with Bradford dye! […]
Terrific broth (TB media) – why and when to use it & how to prepare it
If you want more protein made, but don’t have a lot of space to scale up, try expressing in TB […]
Phosphate forms (names, pKa’s, etc.), buffers basics, & intuition
Dihydrogen phosphate (H₂PO₄⁻) is monobasic and Monohydrogen phosphate (HPO₄²⁻) is dibasic You can remember this by reasoning through it… Starting […]
OD (Optical Density) vs absorbance in biochemistry & OD600 monitoring of bacterial growth
OD stands for “Optical Density” and it’s a measure of how much light “gets lost” as it passes through something. […]
Things to focus on in biochemistry (my recommendations, at least)
If you want to learn biochemistry, here’s an outline of the things I think are most important to focus on. […]
