Recombinant protein purification workflow Protein chromatography Museum analogy of protein chromatography IMAC (Immobilized Metal Affinity Chromatography) Strep tag purification FPLC: Meet the AKTA! Ion Exchange Chromatography Size Exclusion Chromatography Analytical SEC Recombinant protein expression Overexpression in bacteria with the T7 system Bacmid Expression Vector System (BEVS) aka nsect cell based expression Measuring how much protein you have SDS-PAGE Protein concentrating Measuring protein concentration Flash freezing of your pure protein
