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The Bumbling Biochemist

The Bumbling Biochemist

on a mission to make biochemistry fun and accessible to all

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Lab techniques

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Ways to measure “expression”

SDS-PAGE

Agarose gel electrophoresis

Urea-PAGE

PCR

RT-qPCR

Radiolabeling nucleic acids

spin-column PCR purification (clean-up)

Minipreps (alkaline lysis)

RNA extraction/purification

western blots

other blots

Slot blot

EMSA (electrophoretic mobility shift assay)

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