note: if you’re looking for posts on recombinant protein expression & purification see the Protein expression and purification page; for mammalian cell culture, see Mammalian cell culture page; for more on structural biology, see Structural biology page; for random practical tips and tricks, see Random practical lab tips & tricks page
Gel electrophoresis
Native PAGE (PolyAcrylamide Gel Electrophoresis) & Blue Native PAGE
Blots
Working with nucleic acids
Measuring “expression”
Manipulating “expression”, etc.
Detecting & measuring interactions
There are also many “biophysical” techniques I don’t have dedicated posts on, including:
- surface plasmon resonance (SPR) (which detects interactions with molecules stuck on a chip)
- isothermal calorimetry (ITC) (which measures tiny changes in temperature caused by binding interactions)
- “single molecule” methods that use high resolution fluorescence microscopy or other techniques to track interactions
Molecular cloning
Measuring concentrations
Experimental design & considerations
Another fundamental structural biology technique I don’t have a dedicated post on is NMR (nuclear magnetic resonance) – it’s basically a molecular MRI. It’s a solution phase experiment (so you don’t freeze your samples in place) that can work well for small floppy things, but requires lots of sample.
