Techniques for buffer exchange (Desalting columns, Dialysis, & Diafiltration): Pros, cons, & going-ons
The 3 D’s of buffer exchange! There are 3 main techniques for buffer exchange of proteins (or nucleic acids): Desalting […]
Demystifying (and de-mythifying) grad school – things to know about what it is and isn’t
Part of my goal with some of the work I do is to de-mystify the hidden curriculum aspects of academic […]
Scientific writing is an iterative, often collaborative and back-and-forth, process – here’s some advice
Extra note for grad school students: One of the key things to know is that a lot more gets to […]
ResolutIon in protein chromatography – what it is & how to get it: selectivity & efficiency
If you want to know more, I recommend checking out Cytiva’s chromatography handbooks. More on HIC and optimizing it: https://www.cytivalifesciences.com/en/us/insights/optimizing-hydrophobic-interaction-chromatography […]
The future of structural biology is likely dynamic
Proteins don’t have “a” structure – they typically have many; because proteins don’t stay still! Conformational changes (shape-shifts) can come […]
Who’s who (position-wise) in academic science labs
Who’s who in a lab & what do they do? (way overgeneralized); (in academia mainly – makeups, precise roles & […]
Aptamers and a quick comparison of small nucleic acid tools/therapeutics
Small nucleic acid therapeutics/tools (sometimes referred to as oligonucleotide therapeutics) include aptamers; RNAi-acting small RNAs – microRNA (miRNA) and small interfering […]
Gene-related jargon: exons, introns, UTRs, pre-mRNA, mature mRNA, CDS (coding sequence), & cDNA
We often say we stick the “gene” for making a protein into cells to get them to make the protein […]
Aminosäuren
Huge thanks to Ivana Radosavljević, immunotherapy scientist, and self-proclaimed science enthusiast for this translation into German!
