HEK 293 cells – types & uses Cell detachment with trypsin/EDTA: the biochemistry going on when you take cells off! Mammalian cell culture media basics Splitting (aka passaging, subculturing) cells – what, why, & how Trypan blue dye exclusion test in cell counting Phenol red and pH buffering of cell culture media Why you shouldn’t *routinely* use antibiotics in mammalian cell culture, and when you *should* use them Cryopreserving cell stocks Harvesting cell culture Homogenization and lysis (cellular disruption) – an overview of methods and practical considerations Edge effects in multiwell plates
