I don’t have text for you today – I just really wanted to geek out about cell-free protein expression and especially the PURE (Protein synthesis Using Reconstituted Elements) system. So here’s an overview of cell-free expression (lysates vs. PURE etc.) and a deep dive into the journal article behind the PURE system.

Here are a few links and figures

here’s that key paper: Shimizu, Y., Inoue, A., Tomari, Y. et al. Cell-free translation reconstituted with purified components. Nat Biotechnol 19, 751–755 (2001). https://doi.org/10.1038/90802 

And the corresponding News & Views: Swartz, J. A PURE approach to constructive biology. Nat Biotechnol 19, 732–733 (2001). https://doi.org/10.1038/90773 

And that link to the RF paper: Graf, M., Huter, P., Maracci, C. et al. Visualization of translation termination intermediates trapped by the Apidaecin 137 peptide during RF3-mediated recycling of RF1. Nat Commun 9, 3053 (2018). https://doi.org/10.1038/s41467-018-05465-1

more on translation: https://bit.ly/proteintranslationwedding & https://youtu.be/XFNg6OlKWvw

more on His-tag purification: http://bit.ly/histagpurification ; https://youtu.be/JpWJraFcFp4

more on Hildegard Lamfrom: http://bit.ly/hildegardlamfrom 

more on creatine & energy: http://bit.ly/creatineatp ; YouTube: https://youtu.be/IKpI-Nu-LB0

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