We’ve noticed some pH-dependent changes in our protein, so a free web-based tool that I’ve been exploring is H++. It […]

Fluorescence Thermal Shift (FTS), aka Differential Scanning Fluorimetry (DSF) aka Fast Thermal Melt, is a way to measure the thermal […]

Auto-induction is an alternative to IPTG-based induction for genes under regulation by a lac promoter. The media involves a mix […]

RT-qPCR (reverse transcription quantitative PCR) lets you measure “gene expression” at the level of mRNA. Basically, you can see how […]

I can’t believe it’s (almost) been a full academic year as a visiting professor at St. Mary’s College of California! […]

Page navigation: Videos; Graphics; Key points; Related posts; Recommended reading Videos Lipid anabolism (synthesis) Lipid catabolism (breakdown) Integrated lipid metabolism […]

Don’t be fooled by biochemically-plausible-sounding claims! Use biochemistry knowledge with caution! Seek out systematic reviews & meta-analyses But remember: garbage […]

The biggest thing is to not be intimidated by them! Instead, after looking at the “start” and the “end” of […]

At least some of the many ways… Ethanol metabolism is practically unregulated, but it produces metabolites (e.g. NADH & ATP) […]

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