Urea PAGE and comparison to SDS-PAGE
Today, I brought out the big gels! And, although they might look like they’re just scaled-up versions of the SDS-PAGE […]
Balancing chemical equations
You put some molecules in, you get some molecules out, you count the atoms within to be sure they balance […]
Measuring protein concentration – the many ways…
Today I finished a couple of protein purifications that I started yesterday, and I wanted to figure out what my […]
Protein cleaning – recombinant protein expression and chromatography purification workflow
You’ve gotta make a mess to get proteins clean! I do a ton of these “cleanings” (protein purifications) because as […]
Welcome to Wikipedia editing
Wikipedia is free to edit, so we each can play a part, so today, as I work on 2 articles […]
Cryo-electron microscopy (cryo-EM) & structural biology
Dying to know “What’s the deal with cryo?” Cryo-Electron Microscopy (CryoEM) has transformed the structural biology field in recent years, […]
western blot workflow
SDS-PAGE lets us separate proteins by size, but doesn’t tell us what the proteins we separated are. If you add […]
Agarose gel electrophoresis
AGAROSE GEL ELECTROPHORESIS provides a way to separate differently-sized pieces of DNA! With the start of the new year, many […]
Candles, combustion, and waxes
I’m finally getting around to de-decorating and, putting away Hanukkah candles, I realized that because Hanukkah fell during #20DaysOfAminoAcids, I […]
Fermentation & leavening
I was watching one of those “2020 in Review” TV specials and it was talking about how a lot of […]
