Downloadable guide
Overview graphics
YouTube playlists
- Molecular cloning: https://youtube.com/playlist?list=PLUWsCDtjESrESdmbna9aUt-VXwaQWMONu
- Recombinant protein expression: https://youtube.com/playlist?list=PLUWsCDtjESrE8V74DhA9VwitFz8PBJ5pu
- Recombinant protein expression: https://youtube.com/playlist?list=PLUWsCDtjESrE8V74DhA9VwitFz8PBJ5pu
- Protein purification: https://youtube.com/playlist?list=PLUWsCDtjESrEtqQkEXsQpTyRfMhywBVQ7
- Lab techniques and equipment: https://www.youtube.com/playlist?list=PLUWsCDtjESrGlFUCaizromCCk1-z2-GMQ
Protein chromatography
Protein purification often involves a series of sequential column purifications that take advantage of different properties of different proteins
Recombinant protein expression
Homogenization and lysis (cellular disruption) – an overview of methods and practical considerations
Measuring protein concentration – emphasis on Bradford & UV-based methods
Now what?
For posts on playing with proteins (erm, I mean biochemistry techniques you can use to study proteins), see the Lab Techniques page.
If you want to get a look at the proteins at the atomic level, see the Structural Biology page.
