Ribosome-focused methods: ribosome profiling/footprinting/Ribo-Seq; polysome profiling; toeprinting
The biochemical equivalent of Disneyland trying to figure out how popular a ride is is scientists trying to figure out […]
My postdoc work!
My postdoc work – It’s here!!!!! PS – we originally posted it as a pre-print (paper that hasn’t yet been […]
Want to be a scientist? Start making (and keeping up to date) a CV (Curriculum Vitae) early!
That’s a lot to cram in, so you want to keep out unnecessary “filler” words and full sentences (e.g., “I […]
Tips for giving a research presentation (especially to a more general audience)
When you give a research presentation* (especially to a more general audience), you’re telling a story. And you’re the director […]
Predicting protein protonation states with H++
We’ve noticed some pH-dependent changes in our protein, so a free web-based tool that I’ve been exploring is H++. It […]
Differential Scanning Fluorimetry (DSF), aka Fluorescence Thermal Shift (FTS), aka Fast Thermal Melt
Fluorescence Thermal Shift (FTS), aka Differential Scanning Fluorimetry (DSF) aka Fast Thermal Melt, is a way to measure the thermal […]
Skills for aspiring scientists (such as people wanting to go to grad school) to develop early on
So you think you want to be a scientist? Maybe you’re an undergrad or postbac hoping to go to grad […]
Autoinduction for recombinant protein overexpression
Auto-induction is an alternative to IPTG-based induction for genes under regulation by a lac promoter. The media involves a mix […]
Notes on RT-qPCR vs RNA-seq
RT-qPCR (reverse transcription quantitative PCR) lets you measure “gene expression” at the level of mRNA. Basically, you can see how […]
One-year teaching update
I can’t believe it’s (almost) been a full academic year as a visiting professor at St. Mary’s College of California! […]
