Organize things in the way that works best for you – just start early and stay consistent!

  • Establish a consistent naming and organization scheme for your files
    • Include date in file names
      • Save multiple (dates) drafts to go back to things you cut out, etc.
  • save early and often – to the cloud
    • Regularly back up your laptop to an external drive

Keep detailed daily lab notes, organized by date

  • Make sure you have your notes for the day written (or at least jotted down) before you go to bed (do it while things are still fresh)
  • take pictures of physical notes and save them digitally

Additionally, keep easily accessible (in a folder or notebook tab or file – whatever works) things you need often:

  • buffer recipes
  • important coding snippets 
  • a “cheat cheat” of things you often forget
  • protocols
  • experimental figures (see below)
  • Make experimental figures as you go
    • Keep those figures in a figures or results tab, organized by experiment type and/or project

Experiment “easily”

  • Write detailed experimental protocols that you can easily access
    • each time you go to do the experiment, make a copy of the protocol and edit & annotate with the particulars of that specific instance of doing it
      • I like to print out a copy of it and magnet it to the lab bench, then write on it any deviations from the protocol, measurements, etc.
        • then I tape into my physical lab book, take a picture for my notes pictures folder, and type it up in my electronic notebook
    • when possible, figure out your calculations at least the day before and review in the morning
  • Make spreadsheets for calculating master mixes
  • Bookmark key website conversion tools and things

Keep track of what you have

  • maintain (up-to-date) inventories of:
    • primer & any other oligo sequences
    • plasmids, with information about size, antibiotic resistance
      • when relevant: protein tag?, protein size & pI (with and without tag)
    • cell pellets, protein preps, etc.

Plan ahead as much as possible… 

  • longer-term/bigger-picture thinking, periodically (every couple weeks or so) make a to do list and a project status summary 
  • day to day, plan out experiments thoroughly at least the day before
    • if you have multiple things to do, plan how you can stagger, etc.

…but stay flexible because science doesn’t always like to follow your schedule – experiments might not work, the machine might give you an error code, etc. – and meetings tend to magically materialize

Stay up to date with what others are doing

  • Set up alerts (through Google Scholar, PubCrawler, PubMed, etc.) and RSS feeds to stay up to date on the literature
    • I use the RSS reader NewsBlur
  • Regularly check the “online now” sections of websites for key journals of interest
  • Use a reference manager (Mendeley, Zotero, ReadCube, etc.) to save and organize it all

link to video in case embed isn’t working: https://youtu.be/l6WqL0RKBXg 

more on saving the date: https://youtu.be/vFg3tO5C4dQ  

more on plasmid inventorying: https://bit.ly/plasmid_inventory ; YouTube: https://youtu.be/Zknk4zNLAQ8  

You can download my template here: https://docs.google.com/spreadsheets/d/1eMbZUuWlqO-CEYcR445ZaIHUnZYg2uwq/edit?usp=sharing&ouid=110501489579733284162&rtpof=true&sd=true 

more on protein inventorying: https://bit.ly/protein_inventorying ; YouTube: https://youtu.be/myoxFfKpyP8 

here’s a link to my example protein prep inventory template spreadsheet: https://docs.google.com/spreadsheets/d/1Qys8OEK-JlTD8kUvHUN0QsoBG_Gh1t3b/edit?usp=sharing&ouid=110501489579733284162&rtpof=true&sd=true  

more on master mixes: http://bit.ly/mastermixesmultichannels & https://youtu.be/QQiaiERyyGo 

more on alerts & RSS feeds: blog form: https://bit.ly/science_alerts_RSS_feeds  ; YouTube: https://youtu.be/jG3hImJ7fRY   

more on reference managers: https://bit.ly/referencesreference ; YouTube: https://youtu.be/VIds4W2nut8 

more on Adobe Illustrator: https://bit.ly/adobe_illustrator_fundamentals & https://youtu.be/JdVSeZajq6A & https://drive.google.com/file/d/1C72zWcht0gmtRV45Yl1p0xML4JwzFrgp/view?usp=share_link 

link to this and other guides, tip sheets, etc. https://bit.ly/bumbling_uploads 

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