Organize things in the way that works best for you – just start early and stay consistent!
- Establish a consistent naming and organization scheme for your files
- Include date in file names
- Save multiple (dates) drafts to go back to things you cut out, etc.
- Include date in file names
- save early and often – to the cloud
- Regularly back up your laptop to an external drive
Keep detailed daily lab notes, organized by date
- Make sure you have your notes for the day written (or at least jotted down) before you go to bed (do it while things are still fresh)
- take pictures of physical notes and save them digitally
Additionally, keep easily accessible (in a folder or notebook tab or file – whatever works) things you need often:
- buffer recipes
- important coding snippets
- a “cheat cheat” of things you often forget
- protocols
- experimental figures (see below)
- Make experimental figures as you go
- Keep those figures in a figures or results tab, organized by experiment type and/or project
Experiment “easily”
- Write detailed experimental protocols that you can easily access
- each time you go to do the experiment, make a copy of the protocol and edit & annotate with the particulars of that specific instance of doing it
- I like to print out a copy of it and magnet it to the lab bench, then write on it any deviations from the protocol, measurements, etc.
- then I tape into my physical lab book, take a picture for my notes pictures folder, and type it up in my electronic notebook
- I like to print out a copy of it and magnet it to the lab bench, then write on it any deviations from the protocol, measurements, etc.
- when possible, figure out your calculations at least the day before and review in the morning
- each time you go to do the experiment, make a copy of the protocol and edit & annotate with the particulars of that specific instance of doing it
- Make spreadsheets for calculating master mixes
- Bookmark key website conversion tools and things
Keep track of what you have
- maintain (up-to-date) inventories of:
- primer & any other oligo sequences
- plasmids, with information about size, antibiotic resistance
- when relevant: protein tag?, protein size & pI (with and without tag)
- cell pellets, protein preps, etc.
Plan ahead as much as possible…
- longer-term/bigger-picture thinking, periodically (every couple weeks or so) make a to do list and a project status summary
- day to day, plan out experiments thoroughly at least the day before
- if you have multiple things to do, plan how you can stagger, etc.
…but stay flexible because science doesn’t always like to follow your schedule – experiments might not work, the machine might give you an error code, etc. – and meetings tend to magically materialize
Stay up to date with what others are doing
- Set up alerts (through Google Scholar, PubCrawler, PubMed, etc.) and RSS feeds to stay up to date on the literature
- I use the RSS reader NewsBlur
- Regularly check the “online now” sections of websites for key journals of interest
- Use a reference manager (Mendeley, Zotero, ReadCube, etc.) to save and organize it all
link to video in case embed isn’t working: https://youtu.be/l6WqL0RKBXg
more on saving the date: https://youtu.be/vFg3tO5C4dQ
more on plasmid inventorying: https://bit.ly/plasmid_inventory ; YouTube: https://youtu.be/Zknk4zNLAQ8
You can download my template here: https://docs.google.com/spreadsheets/d/1eMbZUuWlqO-CEYcR445ZaIHUnZYg2uwq/edit?usp=sharing&ouid=110501489579733284162&rtpof=true&sd=true
more on protein inventorying: https://bit.ly/protein_inventorying ; YouTube: https://youtu.be/myoxFfKpyP8
here’s a link to my example protein prep inventory template spreadsheet: https://docs.google.com/spreadsheets/d/1Qys8OEK-JlTD8kUvHUN0QsoBG_Gh1t3b/edit?usp=sharing&ouid=110501489579733284162&rtpof=true&sd=true
more on master mixes: http://bit.ly/mastermixesmultichannels & https://youtu.be/QQiaiERyyGo
more on alerts & RSS feeds: blog form: https://bit.ly/science_alerts_RSS_feeds ; YouTube: https://youtu.be/jG3hImJ7fRY
more on reference managers: https://bit.ly/referencesreference ; YouTube: https://youtu.be/VIds4W2nut8
more on Adobe Illustrator: https://bit.ly/adobe_illustrator_fundamentals & https://youtu.be/JdVSeZajq6A & https://drive.google.com/file/d/1C72zWcht0gmtRV45Yl1p0xML4JwzFrgp/view?usp=share_link
link to this and other guides, tip sheets, etc. https://bit.ly/bumbling_uploads
