• Resuspend in: TE (10 mM Tris, pH 7.5 to 8.0, 1 mM EDTA); Tris (10 mM Tris-HCl, pH 8.0); or molecular biology grade water
  • add 1μL per nmol (look on tube) for 1mM or 10μL per nmol for 100μM
• make dilutions of the stock to your working concentration & aliquot if you’re going to be using lots of times
  • avoids contaminating stock, minimizes freeze-thaws, lets you keep at higher concentration which is more stable
• don’t do huge dilutions – instead dilute step-wise

more in video & figures and these great web pages I found

IDT Tips for resuspending and diluting your oligonucleotides https://www.idtdna.com/pages/education/decoded/article/tips-for-resuspending-and-diluting-your-oligonucleotides Nolan Speicher, former IDT associate. Published Mar 31, 2017 Revised/updated Sep 22, 2017

My oligos have arrived: Now what? Resuspension, dilution, storage, and other tips https://www.idtdna.com/pages/education/decoded/article/my-oligos-have-arrived-now-what- Elisabeth Wagner, PhD, Manager of Scientific Applications Support, IDT. Published Jan 14, 2014 Revised/updated Sep 10, 2016

Storing oligos: 7 things you should know https://www.idtdna.com/pages/education/decoded/article/storing-oligos-7-things-you-should-know Nan Pazdernik, PhD, Science Writer, IDT Nolan Speicher, former IDT associate. Published Jun 20, 2017 Revised/updated Dec 3, 2020

Millipore Sigma: Oligonucleotide Handling & Stability: https://www.sigmaaldrich.com/US/en/technical-documents/protocol/genomics/dna-and-rna-purification/oligonucleotide-handling-and-stability  

more on concentrations and C1V1=C2V2: blog form: http://bit.ly/c1v1equalsc2v2   ; YouTube: https://youtu.be/JbtTwDOVyOo 

more on dimensional analysis: http://bit.ly/dimensionalanalysising & https://youtu.be/KQMA0aAGfP4

more on how these things are synthesized: http://bit.ly/solidstateoligo