People really seemed to like yesterday’s video, and I figured it’s good teaching practice for my ultimate goal of being an undergrad-focused professor, so today I made a quick (again, unpolished) one on SDS-PAGE, a technique we use to separate proteins by size (technically length) by unfolding them, coating them in a detergent (artificial soap) called SDS, and sending them traveling through a PolyAcrylamide Gel mesh using Electrophoresis). I didn’t have time to go into as much detail as I wanted because of length constrictions, but if people want I can do a follow up where I talk more about staining and western blotting (how you can use little protein-of-interest-specific proteins to probe for specific proteins after the run, since SDS-PAGE will only tell you about relative size & quantity of proteins, not what they actually are). But for now, it’s back to work for me.
And if you want a written & graphics version: http://bit.ly/sdspageruler
The shut up and let me just see an animation version: