Crystal harvesting and CryoLoop preparation & recycling
Going meta and retro made a video of a Twitter thread I made in 2018 and posted on my blog […]
Protein crystallization – Give your protein a nice cocktail!: precipitants, crystallization diagrams, screening and optimization
I screen, you screen, we (probably not all) screen precipitants and pH in hopes protein crystals will be seen! Protein crystallography aims […]
Intro to protein crystallization (the actually getting crystals stuff and why it’s so hard)
One time I shipped some protein crystals and on the shipping form it asks what’s contained in there and what […]
Using the PDB to investigate x-ray crystallography structures: resolution, R-factor, outliers & more
A guide to interpreting, analyzing, and playing with structures in the Protein Data Bank (PDB), with an emphasis on x-ray […]
Where’s the protein?! Troubleshooting and optimizing recombinant protein expression
Today I thought I’d go over some background and practical advice for things you can do if you ask a […]
Jane Richardson – crystallographer and ribbon diagram inventor
Jane Richardson invented the ribbon representation for protein structures. You know what I’m talking about even if you have no […]
HDX-MS: Hydrogen Deuterium eXchange – Mass Spectroscopy
One of the things I’ve been feeling down about lately is that I’ve been waiting for an HDX-MS dataset – […]
The PDB (Protein Data Bank)
Happy 50th birthday to the PDB! 50 years since its founding, The Protein Data Bank continues to be, *the* place […]
Rosalind Franklin and Photo 51
Rosalind Franklin – here’s a woman who definitely deserves to be celebrated during Women’s History Month (and year round!) You’ve […]
Flash freeze dance – cryoprotectants, vitrification, crystallization, etc.
Flash freeze dance time! I love protein purification, but I always have this weight of worry that – no matter […]
