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The Bumbling Biochemist

The Bumbling Biochemist

on a mission to make biochemistry fun and accessible to all

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Category: 365 Days of Science

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Practical protein purification tip: easily & quickly monitor protein elution by eye using Bradford dye

July 30, 2023July 30, 2023

Quick protein purification tip: when doing gravity flow chromatography, monitor protein elution by dye by checking fractions with Bradford dye! […]

Terrific broth (TB media) – why and when to use it & how to prepare it

July 24, 2023July 24, 2023

If you want more protein made, but don’t have a lot of space to scale up, try expressing in TB […]

Phosphate forms (names, pKa’s, etc.), buffers basics, & intuition

July 23, 2023July 24, 2024

Dihydrogen phosphate (H₂PO₄⁻) is monobasic  and  Monohydrogen phosphate (HPO₄²⁻) is dibasic You can remember this by reasoning through it…  Starting […]

OD (Optical Density) vs absorbance in biochemistry & OD600 monitoring of bacterial growth

July 17, 2023July 18, 2023

OD stands for “Optical Density” and it’s a measure of how much light “gets lost” as it passes through something. […]

Things to focus on in biochemistry (my recommendations, at least)

July 16, 2023August 24, 2025

If you want to learn biochemistry, here’s an outline of the things I think are most important to focus on. […]

Labbing (and teaching) at a small undergrad college (as opposed to a research-intensive institution)

July 5, 2023January 24, 2026

People have asked me if I will miss the lab and the truth is one of the reasons I’ve wanted […]

Prof Bri’s “Office Hours”

July 3, 2023July 5, 2023

I have an office! And, in addition to physical office hours for my students here at St. Mary’s College of […]

Step up to the microplate! But which one?

June 28, 2023June 30, 2023

The colors aren’t just to look stylish… Some plates have transparent bottoms but opaque sides – these can be used […]

Practical Python Practice Problems for People who Pipet

June 27, 2023June 27, 2023

Learning to code is much more fun – and useful – if you practice with things that are practical for […]

Tips for increasing your PCR specificity (decreasing nonspecific product formation)

June 25, 2023June 25, 2023

When it comes to PCR, the thing I typically care most about is specificity. I want my sequence of interest […]

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