I’m crossing protein-protein crosslinking off of my biochemistry bucket list. And thought I’d share what I’m learning. So if you’ll allow me to geek out a bit (sorry I don’t have text but I will post some links and will probably make a longer more detailed formal form later. I just like to talk about things I’m excited about)

There’s a lot more you can do with all sorts of different types of crosslinking and conjugation. Here are some good resources to learn more:

MIT Open Courseware R5. Overview of Cross-Linking, Including Photo-Reactive Cross-Linking Methods, Elizabeth Nolan https://youtu.be/_Rcd-NZwoi4 

ThermoFisher has a ton of info on their website and handbooks

Crosslinking Protein Interaction Analysis: https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/pierce-protein-methods/crosslinking-protein-interaction-analysis.html 

Crosslinking Reagents Technical Handbook: https://tools.thermofisher.com/content/sfs/brochures/1602163-Crosslinking-Reagents-Handbook.pdf 

Bioconjugation technical handbook: http://assets.thermofisher.com/TFS-Assets/BID/Handbooks/bioconjugation-technical-handbook.pdf 

For more on the structural side: 

O’Reilly, F.J., Rappsilber, J. Cross-linking mass spectrometry: methods and applications in structural, molecular and systems biology. Nat Struct Mol Biol 25, 1000–1008 (2018). https://doi.org/10.1038/s41594-018-0147-0 

more on cryo-EM: https://bit.ly/cryoEMbumblyintro ; video on YouTube: https://youtu.be/qcS6oZlEtH0 

more about all sorts of things:  #365DaysOfScience All (with topics listed) 👉 http://bit.ly/2OllAB0 or search blog: https://thebumblingbiochemist.com    


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