How SPRI beads (SPRIselect, AMPure, etc.) work for nucleic acid clean-up & size selection
SPRI (Solid-Phase Reversible Immobilization) beads are used frequently in the preparation of DNA or RNA sequencing libraries. The protocols for […]
My favorite resources for learning pandas (python library for analyzing datasets in table formats)
I highly recommend learning pandas (the Python data analysis library) if you want to do any sort of data analysis […]
You never out-grow textbooks! (Only your ego can!) – and here’s one of my favorites…
People ask me all the time for textbook recommendations and my favorite biochemistry textbook of all time is Lehninger’s Principles […]
A lot to love about aliquots!
Aliquots are like snack-size portions – they’re smaller portions of something you have a lot of. This could be something […]
Gel electrophoresis types & choices
The basic idea of any form of gel electrophoresis is the same (separate molecules by size by using electricity to […]
Sample loading buffers for gel electrophoresis
Buffer you load a gel you have to mix it with a sample loading buffer! These buffers have a few […]
My watch and listen to lists: biochemistry (and more) seminar series, recorded courses, podcasts, etc. I watch/listen to
My biochemistry watchlist – some suggestions for biochemistry, biology, and chemistry seminar series, recorded courses, etc. YouTube: https://youtu.be/R7btBytjKFA There’s a […]
Practical tips for pipetting small volumes
There’s lots to say about pipetting little amounts! Firstly, if you don’t have to, don’t! Avoid it altogether! Don’t pipet […]
Intrinsically Disordered Regions (IDRs), Intrinsically Disordered Proteins (IDPs), and IUPred scores & plots
We often think about proteins as being these beautifully-shaped things, composed of spiraling alpha-helixes and accordion-like sheets of beta-strands. And […]
The hydropathy index and hydropathy plots
Don’t be scared off by a hydropathy plot! It can help you predict membrane-spanning regions of proteins and more – […]
