Adenovirus (Ad) vectors are largely gutted-out versions of a type of double-stranded DNA virus that typically remains episomal (doesn’t integrate with the genome) and when it does integrate, it tends to be at okay places. A couple of key benefits are they can deliver large genetic payloads (>30 kb) and can transduce dividing & non-dividing cells. However, they are highly immunogenic – can be good for vaccines and cancer treatments, but bad for most gene therapies.
