About

On Twitter and Facebook, I'm taking walking people through some of the protein biochemistry work I do, using  and I'll be re-posting the Facebook posts here. To make it easier to follow along, I'm posting in experimental steps not real time. Feel free to ask questions and call me out if I slip into jargon!

Most Recent Post

Urea-PAGE gels, made to order!👩‍🍳 Check out our menu! 💁‍♀️ When we run SDS-PAGE gels to separate proteins by size, we...

Posted by Brianna Bibel on Wednesday, October 10, 2018

Past Posts

January 2018

Happy New Year! This year, I'm trying to do #365DaysofScience - each day I will post a picture of something science-y...

Posted by Brianna Bibel on Monday, January 1, 2018

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The broth I showed you yesterday was clear because it was sterile. At that point cloudy = 😧 Now the media is cloudy because the bacteria multiplied overnight, producing more & more of my DNA. I used antibiotic selection again, so now cloudy = 😀 #365DaysofScience

Posted by Brianna Bibel on Thursday, January 11, 2018

I spin down the cultures in a centrifuge -> 1st pelletThen I lyse (break open) the cells and precipitate out the...

Posted by Brianna Bibel on Friday, January 12, 2018

‪My #365DaysofScience is coming to you from @CSHL, where I’m a grad student, & where my mom is visiting for the weekend 🤓. So while my cells are growing & my mom & I are having fun, enjoy the view! ‬

Posted by Brianna Bibel on Saturday, January 13, 2018

‪After purifying the bacmid containing the gene for my protein, I put the bacmid into insect cells in a process called...

Posted by Brianna Bibel on Sunday, January 14, 2018

My mom's visiting, so I asked her to help me take pictures for #365DaysOfScience. She took her job very seriously, so today I bring you, not 1, but 2 pictures of me washing flasks! 😆

Posted by Brianna Bibel on Monday, January 15, 2018

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After allowing the cells to express my protein for several days, I spin down the cell suspension in a centrifuge to separate the cells from the growth media #365DaysofScience

Posted by Brianna Bibel on Friday, January 19, 2018

While the insect cell cultures are spinning, I finish preparing a resuspension buffer. I keep a concentrated stock of...

Posted by Brianna Bibel on Saturday, January 20, 2018

After 15 minutes of high-speed spinning, the insect cells (with my protein inside) are separated from the growth media....

Posted by Brianna Bibel on Sunday, January 21, 2018

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I had to pellet my cells in 6 bottles because I had so much, but it's all the same protein construct being expressed, so...

Posted by Brianna Bibel on Tuesday, January 23, 2018

As promised, my favorite part of harvesting insect cells - flash freezing the pellets in liquid nitrogen! #365DaysofScience

Posted by Brianna Bibel on Wednesday, January 24, 2018

After a swim in liquid nitrogen, the cell pellets are fully frozen & ready to go into the -80°C freezer to make sure...

Posted by Brianna Bibel on Thursday, January 25, 2018

When it's time to start a protein purification, the first step is to lyse (break open) the cells they're in. After...

Posted by Brianna Bibel on Friday, January 26, 2018

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February 2018

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March 2018

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Protein concentrators come in many sizes & molecular weight cut-offs (MWCO). You put your sample in the top chamber &...

Posted by Brianna Bibel on Wednesday, March 7, 2018

Once my protein is concentrated to the desired volume/concentration, it's time to head to the size exclusion column...

Posted by Brianna Bibel on Thursday, March 8, 2018

What did I mean by "sample loop?" The sample loops are basically just pieces of tubing that come in different sizes....

Posted by Brianna Bibel on Friday, March 9, 2018

Size exclusion chromatography (SEC), as you might have guessed from the name, separates components based on their size....

Posted by Brianna Bibel on Saturday, March 10, 2018

Size exclusion chromatography (SEC) resolution depends on the size of the beads and the sizes of the beads' pores. The...

Posted by Brianna Bibel on Sunday, March 11, 2018

The run method is simple for the size exclusion column (SEC) - I only have a single buffer running at a constant rate,...

Posted by Brianna Bibel on Monday, March 12, 2018

One of the most important (though also the longest & most boring) parts of size exclusion chromatography (SEC) is the...

Posted by Brianna Bibel on Tuesday, March 13, 2018

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April 2018

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May 2018

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June 2018

Because, as I’ve 💬, the silver stain is SO sensitive, even w/super clean water 💦 & super clean staining 📦 you still need...

Posted by Brianna Bibel on Friday, June 1, 2018

Forming formaldehyde solution to form a silver image of proteins in our SDS-PAGE gel! 🤓🤞 it performs! 😬_While our gel...

Posted by Brianna Bibel on Saturday, June 2, 2018

A silver stain without silver isn’t even a stain. 🤷‍♀️Formaldehyde is powerful, but it’s not magical 🧙‍♂️(or alchemical)...

Posted by Brianna Bibel on Sunday, June 3, 2018

That pH lowering chemical I mentioned yesterday❓🥁 It’s nothing fancy 🤷‍♀️ in fact, it’s often used as a water softener....

Posted by Brianna Bibel on Monday, June 4, 2018

Let’s meet our final silver stain ingredient 👤: tungstosilicic acid (TSA). 👉 TSA serves as a CARRIER molecule 🚚 👉 it...

Posted by Brianna Bibel on Tuesday, June 5, 2018

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July 2018

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August 2018

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https://www.facebook.com/brianna.bibel/posts/10160691064035487?__xts__[0]=68.ARCUfmg-9rMBW6FntAh_HbaFhhqIn7TM6erGiXUxye2cNRVuOpep4S3pjzIuwTgYGlP81XU7QzTva-nYenq9mD5egs9cxQ9M_clx1bFLjILgV25F7MQiBhnq-98VRYOZQ9wcFCU&__tn__=C-R

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https://www.facebook.com/brianna.bibel/posts/10160699664800487?__xts__[0]=68.ARD_7v5_6V9tyy5GPNBvgcDI4Od05Xubllz8ggSR_PPktt-mpOZ1jlxWVudNAvxm6prtZJb6TS8cKSUf9gAdmCIRihNGl4c9AGvNC3Q3l6McVlBT5iFPFY7PLk80NDAdrdOM-Ns&__tn__=C-R

https://www.facebook.com/brianna.bibel/posts/10160703491965487?__tn__=C-R

https://www.facebook.com/brianna.bibel/posts/10160706843950487?__xts__[0]=68.ARD2-LGioBpCBi55zAmXtGwZoZ_j8H38i-NknWppJdLG4RmOZHu68vMQz3UsntfkPhGya_XT8p0FN7n-mdyTrqvInJOMqpbQnWs7F8ZITcRW2e7hWaxDUVOrTPzp2FA-9Gl9PGQ&__tn__=C-R

https://www.facebook.com/brianna.bibel/posts/10160710563970487?__xts__[0]=68.ARCrJ8CPu40HL38B4WEEC8ckV3j8UaAkaiHcQP06In9Wc80wDj04IzUrsr5G-hBq863JuyVCr2G7MbpJ65EG0PA9FZgG8XNpdZ74ed8yVMUQyY3Hxbkgd0U5jd-7eIfywqmpQRU&__tn__=C-R

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September 2018

https://www.facebook.com/brianna.bibel/posts/10160729049045487?__xts__[0]=68.ARCYcy2jSWG470b9GOmjaDPyyibFCmu6CoRas6Gy5MMgwN2Y-TabQC6_xLKS2boR-8l90XOeLxBPWt9FLGhND92UR9f-6rIe1O1X-5cwUA0VXh2WZIwdu72qBKgxLy3Dq4K34hhLnIRQiRQrSrFnr3kPo1iix9t57vTbCfOPhfzirRgDRxFkyQ&__tn__=C-R

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https://www.facebook.com/brianna.bibel/posts/10160722665315487?__xts__%5B0%5D=68.ARCjvf0Hb8wgyS_HaXclMcJJZneNjQhdFwkCzyhAO_p6hbS2_LzVIF6UbL-RKcBHqWvMgsr2Fopbb2lhhuFy4RECKBEkaqgyNQfLfvvfj2FVjbyPKA2_uJrlNuTXhNMijDTdA5_AHo4WMMgZAeSqTdICSYvCEFRJLTEmPxY2irPahE3dcsgo-g&__tn__=C-R

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Go long! 🏈 This is NOT a *short-pass* OPTICAL FILTER! 🙃_We’ve bound luminescent SYPRO Ruby protein stain to proteins...

Posted by Brianna Bibel on Wednesday, September 5, 2018

A little brighter…🔅🔆 not THAT much! 😬 Don’t SATURATE the SENSOR! ⚠️ The point of staining our SDS-PAGE gel is to 👀 where...

Posted by Brianna Bibel on Thursday, September 6, 2018

I hope you’re learning about SDS-PAGE gels from #thebumblingbiochemist 🤞but what can you (& she) learn from the gels...

Posted by Brianna Bibel on Friday, September 7, 2018

yoU REAlly want to know what’s in here?🕵️‍♀️ This is our RNAse-free room, where we run UREA PAGE gels to separate & 👀 at...

Posted by Brianna Bibel on Saturday, September 8, 2018

Would you like a *side* of phosphate with that base to *tide* you over while you wait to form a phosphodiester bond? 🤔...

Posted by Brianna Bibel on Sunday, September 9, 2018

Just dropping by to ✅ base on nucleic acids (NA) using a NANODROP 🙃 I’m more of a crystal girl myself 💎 but these...

Posted by Brianna Bibel on Monday, September 10, 2018

Ring, ring… ☎️ It’s a PURINE! 🤓 On a coffee break? ☕️ Then you’re one step ahead of me! 👍 We usually 🤔 of purines in the...

Posted by Brianna Bibel on Tuesday, September 11, 2018

This post may be all about that *base* 🎶 & 🤞 you won’t have treble understanding it 🙃 BUT please CUT out that “extra...

Posted by Brianna Bibel on Wednesday, September 12, 2018

U shouldn’t be in DNA! 😳 The “mC” 🎤 let this base sneak in…🙄🙃_Uracil (U)’s 1 of 5 canonical NUCLEOBASES 👉 “unique”...

Posted by Brianna Bibel on Thursday, September 13, 2018

Tying the glycosidic knot -It’s *prime* time for a sugar-nucleobase wedding! After deSIDEing whether to put a ring on...

Posted by Brianna Bibel on Friday, September 14, 2018

The A To Z of AZT 👉 “nuking” HIV with NUCLEOSIDE ANALOGUES 💉-You may have👂of CENTRAL DOGMA of molecular biology 👉 DNA...

Posted by Brianna Bibel on Saturday, September 15, 2018

⏰ to go to the ATP 🏧 to get some cellular energy money 💵 to *tide* these nucleosides over 🤓_NUCLEOTIDES are the...

Posted by Brianna Bibel on Sunday, September 16, 2018

When it comes to DNA-based dating services, NUCLEOTIDE POLYMERIZATION is the real deal! 😍_NUCLEOTIDE monomers link...

Posted by Brianna Bibel on Monday, September 17, 2018

Today was a super productive & exciting day of #lifeinthelab! 🤗👩‍⚕️In addition to a part-time #scicomm “superhero,”...

Posted by Brianna Bibel on Tuesday, September 18, 2018

JANET LITSTER RIDEOUT! One of the scientists credited with discovering that AZT could be used to treat HIV. She also did...

Posted by Brianna Bibel on Wednesday, September 19, 2018

U are invited to A T for 2 🍵 (HYDROGEN BONDS) 📮 C 👀 G if you want to add a 3rd party! 🙃 Touching base on DNA...

Posted by Brianna Bibel on Thursday, September 20, 2018

G·U’s wobble but they don’t fall down! 🙃 But this WOBBLE BASE PAIR in RNA does help keep the number of proteins we need...

Posted by Brianna Bibel on Friday, September 21, 2018

See a DNA double helix, put your right thumb up 👉 If the twist follows your fingers your graphic designer is in luck! 🙃...

Posted by Brianna Bibel on Saturday, September 22, 2018

Getting groovy reading DNA! ☮️ Your DNA’s like a cookbook for making you — including recipes (GENES) for every protein...

Posted by Brianna Bibel on Sunday, September 23, 2018

Where’s the stacking gel?! 🧐 Don’t worry - I didn’t forget it! 😅 This isn’t an SDS-PAGE gel (which I use to look at...

Posted by Brianna Bibel on Monday, September 24, 2018

As a PhD student, not all #365DaysOfScience go well… BUT SOME DO! 🤩 (at least the latter parts of them once you get...

Posted by Brianna Bibel on Tuesday, September 25, 2018

When you want to 👀 RNA, U REAch for UREA! 🤓 We look at proteins using SDS-PAGE & we look at NUCLEIC ACIDS (RNA & DNA)...

Posted by Brianna Bibel on Wednesday, September 26, 2018

Phosphate’s in *charge* ⚡️ of getting our NUCLEIC ACIDS (RNA & DNA) to move ⬇️ through our UREA PAGE gel 🤓_GEL...

Posted by Brianna Bibel on Thursday, September 27, 2018

#TheBumblingBiochemist is running out of energy 😴 so this post will be short, but when we run a UREA PAGE gel to...

Posted by Brianna Bibel on Friday, September 28, 2018

They let me use the big podium 🤓 This morning I gave a talk about “Gender differences in the division of household labor...

Posted by Brianna Bibel on Saturday, September 29, 2018

U REAlly wanna know what happened❓Things can get pretty heated 😡 when running a UREA-PAGE gel to separate NUCLEIC ACIDS...

Posted by Brianna Bibel on Sunday, September 30, 2018

I had an experiment idea 💡 & decided to try it out… I thought I’d messed it up hopelessly 😩 & almost gave up 🤷‍♀️ BUT I...

Posted by Brianna Bibel on Monday, October 1, 2018

TBE or not TBE? 🤔 When running a UREA-PAGE, TBE it shall be! 🙃 “Buffer” you can do Gel Electrophoresis to separate...

Posted by Brianna Bibel on Tuesday, October 2, 2018

October 2018

I had an experiment idea 💡 & decided to try it out… I thought I’d messed it up hopelessly 😩 & almost gave up 🤷‍♀️ BUT I...

Posted by Brianna Bibel on Monday, October 1, 2018

TBE or not TBE? 🤔 When running a UREA-PAGE, TBE it shall be! 🙃 “Buffer” you can do Gel Electrophoresis to separate...

Posted by Brianna Bibel on Tuesday, October 2, 2018

Keep your eye on the dye! 👁 Actually the dyeS 👉 When I run a urea-PAGE gel to separate RNA fragments by size, I use 2...

Posted by Brianna Bibel on Wednesday, October 3, 2018

Reaching out to say hi from #SciOut18! 👋 This awesome science outreach “unconference” (yes, autocorrect, I did mean...

Posted by Brianna Bibel on Thursday, October 4, 2018

After a jam-packed 2 days at the science communication (#scicomm) “unconference” #SciOut18 🌪 it was time to reflect!...

Posted by Brianna Bibel on Friday, October 5, 2018

What’s inside? 🧐 It’s FORMAMIDE! 🤗 Formally removing RNA’s form with formamide! 🤓_When running a UREA-PAGE gel to...

Posted by Brianna Bibel on Saturday, October 6, 2018

I (will hopefully) get by with a lot of help from my friends! Tomorrow I have to give my first in-house seminar....

Posted by Brianna Bibel on Monday, October 8, 2018

I did it! 😅 My 1st “in-house” research presentation (I had to 💬 my research for ~30 min to a room+ful of people from...

Posted by Brianna Bibel on Tuesday, October 9, 2018

Urea-PAGE gels, made to order!👩‍🍳 Check out our menu! 💁‍♀️ When we run SDS-PAGE gels to separate proteins by size, we...

Posted by Brianna Bibel on Wednesday, October 10, 2018